Dye-Affinity Nanofibrous Membrane for Adsorption of Lysozyme: Preparation and Performance Evaluation

Steven Sheng-Shih Wang1small orcid_display_4pp, Szu-Ming Yang1small orcid_display_4pp, Ai Hsin1small orcid_display_4pp and Yu-Kaung Chang1,2*
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1Department of Chemical Engineering, National Taiwan University, 10617 Taipei, Taiwan, ROC
2Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, Taishan Dist., 24301 New Taipei, Taiwan, ROC

Article history:
Received: July 31, 2017
Accepted: October 7, 2017

Key words:
nanofibre, polyacrylonitrile, dye-affinity membrane, lysozyme, adsorption

Polyacrylonitrile (PAN) nanofibrous membrane was prepared by an electrospinning technique. After heat treatment and alkaline hydrolysis, the weak ion exchange membrane was grafted with chitosan molecule and then covalently immobilized with a Cibacron Blue F3GA (CB). Fibre diameter, porosity and pore size of the membrane and immobilized dye density were characterized. Furthermore, the membrane was applied to evaluate the binding performance of lysozyme under various operating parameters (pH, chitosan mass per volume ratio, dye concentration, ionic strength and temperature) in batch mode. The experimental results were directly applied to purify lysozyme from chicken egg white by membrane chromatography. The results showed that the capture efficiency, recovery yield and purification factor were 90 and 87 %, and 47-fold, respectively, in a single step. The binding capacity remained consistent after five repeated cycles of adsorption-desorption operations. This work demonstrates that the dye-affinity nanofibrous membrane holds great potential for purification of lysozyme from real feedstock.

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Paper was presented at the 7th International Forum on Industrial Bioprocessing - IFIBiop 2017, May 21-24, 2017, Wuxi, PR China