doi: 10.17113/ftb.55.01.17.4633 

The Anti-Staphylococcus aureus Effect of Combined Echinophora platyloba Essential Oil and Liquid Smoke in Beef

Zahra Pilevar¹, Hedayat Hosseini^1*, Homa Hajimehdipoor², Farzaneh Shahraz¹, Leyla Alizadeh¹, Amin Mousavi Khaneghah^3* and Maryam Mahmoudzadeh¹

 

¹Food Sciences and Technology Department, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and  Food Technology, Shahid Beheshti University of Medical Sciences, 1981619573 Tehran, Iran
²Traditional Medicine and Material Medical Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti  University of Medical Sciences, 1981619573 Tehran, Iran
³Department of Food Science, Faculty of Food Engineering, State University of Campinas (UNICAMP), Rua Monteiro Lobato 80, 13083-862 Campinas, SP, Brazil

Article history:
Received  February 5, 2016
Accepted  September 16, 2016

Key words:
antibacterial activity, Echinophora platyloba, liquid smoke, Staphylococcus aureus, beef


 

Summary:
In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release. Apart from that, the interactions between these two compounds were measured by the checkerboard assay and by calculating the fractional inhibitory concentration (FIC) indices. Related MIC values of essential oil and smoke were found to be 7200 and 5500 mg/L, and MBC values were 8500 and 8000 mg/L, respectively. The conducted organoleptic assay showed that the addition of 0.05 g of essential oil and 0.6 g of liquid smoke to 100 g of meat samples did not have adverse effect on the overall acceptance. Weaker antibacterial effect against Staphylococcus aureus was observed when only Echinophora platyloba essential oil was used than when it was used in combination with liquid smoke.

*Corresponding author:    This email address is being protected from spambots. You need JavaScript enabled to view it. (H. Hosseini)
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