A Simple and Rapid Determination of ATP, ADP and AMP Concentrations in Pericarp Tissue of Litchi Fruit by High Performance Liquid Chromatography

Hai Liu^1,3, Yueming Jiang¹*, Yunbo Luo² and Weibo Jiang<sup>2

1</sup>South China Botanic Garden, The Chinese Academy of Science, 510650 Guangzhou Leyiju, PR China
²College of Food Science and Nutritional Engineering, China Agricultural University, Qinghuadonglu, 100083 Beijing, PR China
³Graduate School of the Chinese Academy of Science, 100039 Beijing, PR China

Article history:
Received February 25, 2005
Accepted May 25, 2005

Key words:
HPLC, ATP, ADP, AMP, determination, litchi fruit, pericarp tissue

Summary:
A simple and rapid method using high performance liquid chromatography (HPLC) was developed to determine levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in litchi fruit pericarp tissue. This HPLC method used acetonitrile gradient elution and shortened the time required for determinations of adenosine phosphates. This analysis exhibited good repeatability (coefficients of variation 1.28–1.80 %) and recovery rate (94.7–97.1 %). The correlation coefficients of ATP, ADP and AMP with their peak areas at a range of 0–80 ng were 0.9946, 0.9994 and 0.9974, respectively. This method was applied to determine levels of adenosine phosphates in pericarp tissue of litchi fruit at harvest. There were 27.4 μg/g of ATP, 35.4 μg/g of ADP and 7.9 μg/g of AMP on a fresh mass basis.

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