The Conditional Mutation cdc6-1 Affects Chromosome Segregation in Saccharomyces cerevisiae

Aleksandra Comino¹*, Alojz Ihan², Radovan Komel¹ and Carlo V. Bruschi<sup>3

1</sup> National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
²Institute for Microbiology, Medical Faculty, SI-1000 Ljubljana, Slovenia
³Microbiology Group, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, I-34012 Trieste, Italy

Article history:
Received May 12, 2000
Accepted October 26, 2000

Key words:
cdc6-1, chromosome segregation, cell cycle control, nocodazole

Summary:
CDC6 is an essential gene of Saccharomyces cerevisiae involved in the initiation of DNA replication. Interacting with ORC complex of proteins, Cdc6 protein has a pivotal role in loading Mcm proteins on origins of replication. Although much evidence about its function in S phase of the cell cycle is available, only a few data indicate a regulatory function of this protein in G2/M transition phase of the cell cycle. By synchronisation with a microtubule- destabilising drug nocodazole and Fluorescence Activating Cell Sorting (FACS) analysis it was possible to provide evidence that CDC6 is responsible for a proper sequence of genetic events and accurate chromosome segregation during mitosis. 

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