Purification of Yeast Periplasmic Proteins Using Protein Adsorption to Glucan

Renata Teparić, Vladimir Mrša

Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia

Article history:
Received August 3, 2010
Accepted February 3, 2011

Key words:
acid phosphatase, invertase, protein adsorption to glucan

Summary:
Partially purified yeast glucan was used for the one-step purification of acid phosphatase from the Saccharomyces cerevisiae cell extract, as well as for the purification of invertase from several commercially available preparations. In both cases pure enzymes, as judged by electrophoresis, were obtained by the adsorption of impurities to glucan. The method described is simple, provides high yields, causes no significant protein denaturation and is inexpensive. It can most probably be used for other yeast extracellular proteins, as well as for proteins from other sources which have low binding potential for yeast glucan.