Isolation and Characterisation of Bacteriocin 
and Aggregation-Promoting Factor
Production in 
Lactococcus lactis ssp. lactis BGBM50 Strain  

Nemanja Mirkovic1,2, Zorica Radulovic1, Gordana Uzelac2, Jelena Lozo2,3Dragojlo Obradovic1,
Ljubisa Topisirovic2 and Milan Kojic2*

1Faculty of Agriculture, University of Belgrade, RS-11080 Belgrade, Serbia
2Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, RS-11010 Belgrade, Serbia
3Faculty of Biology, University of Belgrade, RS-11000 Belgrade, Serbia

Article history:
Received August 4, 2014
Accepted March 3, 2015

Key words
bacteriocin lactococcin G, aggregation-promoting factor, plasmid curing

Lactococcus lactis ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Žanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.




*Corresponding author:  
   +381 11 3975 960
   +381 11 3975 808

Follow us

 facebook 1 twitter bird_icon LI In Bug


Environmental Policy

sdg publishers compact 4 300x300

QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information