Investigation of Lipase Production by Milk Isolate Serratia rubidaea
Grasian Immanuel*, Palanichamy Esakkiraj, Austin Jebadhas, Palanisamy Iyapparaj and Arunachalam Palavesam
Marine Biotechnology Division, Centre for Marine Science and Technology, M. S. University, Rajakkamangalam, IN-629 502 Tamilnadu, India
Article history:
Received October 23, 2006
Accepted April 3, 2007
Key words:
lipase, casein, soytone, gingily oil, Tween 20, Serratia rubidaea
Summary:
Production of extracellular lipase in submerged culture of Serratia rubidaea has been investigated. The lipase production was optimized in shake flask experiments. The observed pH and temperature range optimum for maximum lipase production were 7–8 and 30–40 °C, respectively. With a selected nitrogen source, casein ((6.5±0.015) U/mL) and soytone ((9.4±0.02) U/mL) were suitable substrates for accelerating lipase production. The optimized concentration of casein and soytone was 24 g/L ((9.95±0.02) U/mL) and 5 g/L ((14.8±0.03) U/mL), respectively. The effect of carbon source on lipase production indicated that starch was suitable substrate to maximize lipase production ((15.60±0.20) U/mL) and the optimum concentration registered was 4 g/L ((17.46±0.20) U/mL). Investigating the effect of lipids and surfactants showed that the gingily oil ((20.52±0.20) U/mL) and Tween 20 ((27.10±0.01) U/mL) were suitable substrates for maximizing lipase production, and the optimum concentrations registered were 15 mL/L ((23.15±0.24) U/mL) and 6 mL/L ((34.20±0.01) U/mL), respectively. Partial purification of lipase indicated that the molecular mass of partially purified enzyme was 54 kDa.
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