Effect of pH, Cultivation Time and Substrate Concentration on the Endoxylanase Production by Aspergillus awamori ZH-26 under Submerged Fermentation Using Central Composite Rotary Design

Yin Li^1,2, Jia Lin¹, Dejing Meng¹, Jian Lu¹*, Guoxian Gu³ and Zhonggui Mao<sup>3

1</sup>Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, 214036 Wuxi, PR China
²Department of Plant Science, North Dakota State University, 58105 Fargo, USA
³School of Biotechnology, Southern Yangtze University, 214036 Wuxi, PR China

Article history:
Received November 8, 2005
Accepted January 17, 2006

Key words:
Aspergillus awamori ZH-26, optimization, response surface methodology, endoxylanase

Summary:
The effect of substrate concentration, pH and cultivation time on the production of endoxylanase from Aspergillus awamori ZH-26 were optimized by statistical analysis using response surface methodology (RSM). Endoxylanase production was optimized by central composite rotary design. Statistical analysis of the results showed that the linear and quadratic terms of these three variables had significant effects. But no interactions between the three variables were found to contribute to the response at a significant level. The optimal conditions were: wheat bran 49.3 g/L, pH=4.14, and cultivation time 103.7 h. Under these conditions, the model predicted an endoxylanase activity of 28.25 U/mL. Verification of the optimization showed that endoxylanase production of 29.65 U/mL was observed under optimal conditions.

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