Stability of Yeast Isoenzyme Profiles in Different Growth Conditions

Sofia Santos¹, Filomena L. Duarte¹ and Célia Pais²*

¹ Estação Vitivinícola Nacional-INIA, 2560 Dois Portos, Portugal
² Centro de Ciências do Ambiente – Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal

Article history:
Received March 17, 1999
Accepted October 20, 1999

Key words:
isoenzyme stability, electrophoretic patterns, yeasts, growth conditions

Summary:
Five isoenzyme systems, namely, esterase, glucose-6-phosphate dehydrogenase, acid phosphatase, alcohol dehydrogenase and lactate dehydrogenase, of yeasts belonging to five different species, Saccharomyces cerevisiae, Torulaspora delbrueckii, Pichia anomala, Schizosaccharomyces pombe and Rhodotorula mucilaginosa were analysed under different growth conditions. Two culture media were used, YEPG and modified grape must. For each medium, comparison of the isoenzyme patterns obtained in exponential and stationary phase of growth was performed. Our results showed that the growth conditions influenced differently the isoenzyme patterns of the studied strains but, under standardised conditions a good stability of patterns was achieved. Different isoenzyme patterns were obtained for the five species under study, confirming the discriminatory power of isoenzyme analysis.

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