Spot-Synthesis: Easy Multiple Preparation of Synthetic Peptides on Cellulose Membrane for the Analysis of Anti-Protein Antibodies
Daniela Lucchesi, Paolo Rovero*
CNR — Istituto di Mutagenesi e Differenziamento, Laboratorio di Sintesi Peptidica, Via Svezia 2A, 56124 Pisa, Italy
Article history:
Received July 9, 1997
Accepted September 30, 1997
Key words:
cellulose paper, epitope mapping, enzyme-linked irnmunosorbent assay (ELISA), multiple peptide synthesis, peptide libraries
Summary:
The SPOT synthesis is a novel technique for the simultaneous multiple synthesis of peptides covalenlly linked to cellulose paper, using conventional solid-phase synthesis strategy. The sets of paper-bound peptides thus obtained can be directly used in solid-phase assays, including ELISA. The technique is a powerful tool for the study of protein-interactions and has been widely applied to antibody characterization and epitope mapping studies. The SPOT technique provides a simple, economic and rapid access to large numbers of short peptide sequences or peptide mixtures which can be tested concomitantly with a single serum and repeatedly with many sera on the same membrane. Recent applications of SPOT synthesis to epitope mapping are reviewed in this report.
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