Citric Acid Production on a Waste Starch Fraction Hydrolysate

Cirila Colnar1, Aleksa Cimerman2 and A. Perdih3

HELIOS, Domžale, Slovenia

2National Institute of Chemistry, Ljubljana, Slovenia
3Department of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia

Article history:

Received January 8, 1994

Accepted April 21, 1994


The waste fraction in wheat starch production, starch B, was hydrolysed by means of liquefying and saccharifying enzymes to prepare a substrate for submerged citric acid fermentation. A suitable Aspergillus niger strain was used. Improved yields could be obtained by growing the inoculum on a starch containing agar. The effect of trace metals in the substrate was reduced by the addition of potassium hexacyanoferrate(II).