getpdf https://doi.org/10.17113/ftb.52.04.14.3701

 

Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum

Marouane Melloul1,2, Driss Iraqi3, MyAbdelaziz El Alaoui1,2, Gilles Erba4, Sanaa Alaoui2, Mohammed Ibriz1 and Elmostafa Elfahime2*

1Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133, 14000 Kenitra, Morocco
2Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientifi c and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat, Morocco
3National Institute of Agronomical Research, Avenue de la Victoire, BP 415, Rabat, Morocco
4Labgene Scientifi c Instruments, Athens Building, Business Park, 74160 Archamps, France

Article history:
Received April 25, 2014
Accepted August 12, 2014

Key words:
cDNA-AFLP, drought stress, transcript-derived fragments, durum wheat, real-time PCR

Summary:
Drought is the single largest abiotic stress factor leading to reduced crop yields. The identification of differentially expressed genes and the understanding of their functions in environmentally stressful conditions are essential to improve drought tolerance. Transcriptomics is a powerful approach for the global analysis of molecular mechanisms under abiotic stress. To identify genes that are important for drought tolerance, we analyzed mRNA populations from untreated and drought-stressed leaves of Triticum durum by cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. Overall, 76 transcript-derived fragments corresponding to differentially induced transcripts were successfully sequenced. Most of the transcripts identified here, using basic local alignment search tool (BLAST) database, were genes belonging to different functional categories related to metabolism, energy, cellular biosynthesis, cell defense, signal transduction, transcription regulation, protein degradation and transport. The expression patterns of these genes were confirmed by quantitative reverse transcriptase real-time polymerase chain reaction (qRT-PCR) based on ten selected genes representing different patterns. These results could facilitate the understanding of cellular mechanisms involving groups of genes that act in coordination in response to stimuli of water deficit. The identification of novel stress-responsive genes will provide useful data that could help develop breeding strategies aimed at improving durum wheat tolerance to field stress.

 

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