doi: 10.17113/ftb.54.03.16.4346 

Correlation Between Phytochemical and Mineral Contents and Antioxidant Activity of Black Glutinous Rice Bran, and Its Potential Chemopreventive Property

Paradorn Ngamdee¹, Uthai Wichai² and Sudarat Jiamyangyuen^1*

¹Department of Agro-Industry, Faculty of Agricultural Natural Resources and Environment, Naresuan University, Muang, Phitsanulok 65000, Thailand
²Department of Chemistry, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000, Thailand

Article history:
Received July 4, 2015
Accepted April 4, 2016

 

Key words:
anthocyanins, black glutinous rice bran, mineral content, antioxidant activity, quinone reductase induction

 

Summary:
In this work total anthocyanin content (TAC), total flavonoid content (TFC), total phenolic content (TPC) and minerals found in five black glutinous rice cultivars (MS, SK, PY, PC and KK) from Thailand were analyzed. The antioxidant activity of anthocyanin-rich black glutinous rice bran extracts against nitric oxide radical (NO˙), superoxide radical (O2˙¯) and lipid peroxyl radical (LOO˙) was also determined. Potential chemopreventive property of rice bran extract was screened based on cellular bioassays for phase II detoxification enzyme induction. Quinone reductase (QR) induction in murine hepatoma cells was used as a marker for this effect. Rice bran extract of cultivar KK had the highest TAC, of SK the highest TFC and of PC the highest TPC. The best antioxidants against NO˙, O2˙¯ and LOO˙ were cultivars KK, MS, and SK, respectively. Overall, TAC, TFC and TPC had a combinatorial effect on the antioxidant activities of all extracts; none of them dominated. Minerals may not play a role in the antioxidant activity of the extracts because most correlations between them and the antioxidant activity were unpredictable. However, rice bran contained high mass fractions of some essential minerals on dry mass basis, including Zn (103–133 μg/g), Se (11–18 μg/g) and Cu (3.8–7.1 μg/g). Chemopreventive study indicated that PC cultivar was the most potent chemopreventor with the lowest concentration of an inducer needed to double the QR activity (CD value) of 0.7 μg/mL. These findings showed that black glutinous rice bran is rich in phytochemicals and some essential minerals, and has a potential chemopreventive property.




 

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