Magnetic Cation Exchange Isolation of Lysozyme from Native Hen Egg White

Magnetic Cation Exchange Isolation of Lysozyme from Native Hen Egg White

Ivo Safarik^1,2*, Zdenka Sabatkova¹, Oldrich Tokar³ and Mirka Safarikova¹¹Department of Biomagnetic Techniques, Institute of Systems Biology and Ecology, Academy of Sciences, Na Sadkach 7, CZ-370 05 Ceske Budejovice, Czech Republic
²Department of General Biology, Faculty of Biological Sciences, University of South Bohemia, Branisovska 31, CZ-370 05 Ceske Budejovice, Czech Republic
³Iontosorb, Anezky Ceske 657/33, CZ-400 07 Usti nad Labem, Czech Republic

Article history:
Received May 2, 2006
Accepted November 23, 2006

Key words:
hen egg white, lysozyme, magnetic separation, cellulose cation exchangers

Summary:
Two magnetic macroporous cellulose cation exchangers (Iontosorb MG CM 100 and Iontosorb MG SHP 100) were used for one-step isolation of lysozyme from native, undiluted hen egg white. Highly purified lysozyme (purity >96 %) with specific activity similar to that of commercial lysozyme preparations was obtained in both cases. Carboxymethyl-based cation exchanger exhibited substantially higher capacity for lysozyme; maximum adsorption capacity was 138 mg/mL. The lysozyme-depleted egg white can be used in the same way as the routinely used egg white because no dilution of this material was necessary during the purification process.

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