Xylanase Production from Trichoderma harzianum 1073 D3 with Alternative Carbon and Nitrogen Sources
Isil Seyis* and Nilufer Aksoz
Hacettepe University, Faculty of Science, Department of Biology (Biotechnology), Beytepe, Ankara, Turkey
Received August 4, 2004
Accepted February 28, 2004
xylanase, Trichoderma harzianum, waste utilization, fruit wastes, molasses
The effect of some natural wastes (orange pomace, orange peel, lemon pomace, lemon peel, apple pomace, pear peel, banana peel, melon peel and hazelnut shell) on the production of xylanase from Trichoderma harzianum 1073 D3 has been studied and maximum activity has been observed on melon peel (26.5 U/mg of protein) followed by apple pomace and hazelnut shell. Also, molasses could be used as an additional carbon source as it decreased the production time approximately by 50 %. Finally, potential alternatives of organic nitrogen source (cotton leaf and soybean residue wastes) were analyzed and it was concluded that peptone could be replaced with these residues especially when economics of the process is the major objective.