Production of Transglutaminase by Streptoverticillium ladakanum NRRL-3191 Grown on Media Made from Hydrolysates of Sorghum Straw

Simón J. Téllez-Luis¹, Juan J. González-Cabriales¹, José A. Ramírez¹ and Manuel Vázquez²*

¹Department of Food Science and Technology, U. A. M. Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Apdo. Postal 1015, Reynosa, 88700 Tamaulipas, Mexico
²Área de Tecnología de los Alimentos, Departamento Química Analítica, Escuela Politécnica Superior, Universidad de Santiago de Compostela – Campus de Lugo, E-27002 Lugo, Spain

Article history:
Received: July 27, 2003
Accepted: February 16, 2004

Key words:
transglutaminase, sorghum, hydrolysates, Streptoverticillium ladakanum

Summary:
The aim of this work was to elucidate the suitability of the biotechnological production of transglutaminase by Streptoverticillium ladakanum NRRL-3191 grown on media made from hydrolysates of sorghum straw. Transglutaminase activity was determined in fermentations on sorghum straw hydrolysates and commercial xylose with initial xylose 10, 20 or 30 g/L. Using media containing commercial xylose 20 g/L, transglutaminase activity up to 0.282 U/mL was obtained in 96 h. Using neutralized, charcoal-treated hydrolysates of sorghum straw with xylose 30 g/L sterilized in autoclave at 121 °C, up to 0.155 U/mL was obtained in 96 h. However, when the sterilization was performed by filtration, using the same hydrolysates with xylose 20 g/L, up to 0.348 U/mL was obtained in 72 h. It was demonstrated that hydrolysates of sorghum straw are suitable media for transglutaminase production by Streptoverticillium ladakanum.

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