Biochemical and Functional Properties of Sardinella (Sardinella aurita) By-Product Hydrolysates

Nabil Souissi*, Ali Bougatef, Yousra Triki-Ellouz and Moncef Nasri

Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d’Ingénieurs de Sfax, Rte Soukra km 5., LP »W«, 3038 Sfax, Tunisia

Article history:

Received December 6, 2005
Accepted June 28, 2006

Key words:

fish protein hydrolysates, heads and viscera, sardinella by-product, functional properties, Sardinella aurita, antioxidant activity


Fish protein hydrolysates (FPHs) with different degrees of hydrolysis (DH of 6.62, 9.31 and 10.16) were prepared from heads and viscera of sardinella (Sardinella aurita) by treatment with Alcalase®. The liquid hydrolysates were spray dried. All spray dried by-product hydrolysates contained 73 to 75 % of proteins and low content of lipids. Reversed-phase HPLC profiles showed that low DH hydrolysates contained high hydrophobic peptides and the extent of hydrolysis resulted in an increase of fish hydrophilic peptides. Some functional properties of FPHs were assessed and compared with those of casein or the undigested sardinella protein. Solubility increased while emulsifying capacity and whippability decreased with the increase in the degree of hydrolysis. The product, with a DH of 10.16 %, had an excellent solubility (100 %) over a pH range of 6.0–10.0. The antioxidant activity of the hydrolysates was also tested. All FPHs exhibited more than 50 % inhibition of linoleic acid peroxidation. The antioxidant activity of FPH with 10.16 % DH was about 73 % of that of a-tocopherol, a natural antioxidative agent.


*Corresponding author: 
                                               ++216 22 95 08 54
                                               ++216 74 27 55 95

Search FTB

Follow us

 facebook 1 twitter bird_icon LI In Bug


QR Code


We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete them, see our privacy policy.

I accept cookies from this site.

EU Cookie Directive Module Information